Then the Renilla luminescence was measured using the luminometer. 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 6. Assay Platforms: ELISA MSD Immulite 1000 We are equipped with advanced instruments such as Molecular Devices M5 Microplate Reader for the classical single. SpectraMax M5e Multi-Mode Microplate Reader Features Patented optimization The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Applications Scientist - Molecular Devices) Application note In collaboration with. Supplier: Molecular Devices. Field. FP assay do not perform well with protein:protein interactions since there are limitations to matching fluorophore lifetime to size of the labeled molecule. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903)The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 40266 Email: drugdiscoverytech@lifetech. HSL_EQ_017: Use and Maintenance of a Laboratory. Contact Molecular Devices technical support for equipment service that requires the removal of covers or panels. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The Spectr. 3465 or email us. This document is provided to customers who have purchased Molecular Devices, LLC (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The system provides automation for up to 50 microplates for easy walkaway automation. Some units include stackers for labs with larger volume requirements. Molecular Devices. Molecular Devices M5 multiformat plate reader (UV/vis absorbance, fluorescence, luminescence, time-resolved fluorescence, fluorescence polarization). The FlexStation 3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. With optimized reagents, option to operate the readers. $87,165. 5. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette. For a higher . Molecular Devices. Unit comes with software and computer. ( Molecular Devices ) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. 3. Molecular Devices microplate readers. A single strong absorbance band between 500–600 nm is an indicator of the formation of AuNPs. 5. The TBO spectrum also was captured by Fourier-transform infrared spectroscopy (FT-IR) (Nicolet 6700, Thermo Fisher Scientific, Waltham, MA, USA). Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Fluorescence Polarization (FP) Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. The donor and acceptor can be used to label a variety of biomolecules, for. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. Prepare your i3/i3x instrument for shipment. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. 1700 +1 800. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. You should get comparable results from top and bottom reads using EC50's to compare reads. With a wavelengthUser Manuals, Guides and Specifications for your Molecular Devices SpectraMax M5 Laboratory Equipment. The SpectraMax M5 features a. 0A-Sunnyvale, CA-Made in USAMolecular Devices SpectraMax® M5. Contact the GSS Team Today!SpectraMax® M5 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. 635. The following document is. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. 1. Quantiferon-TB Gold in Tube Kit. NORTH AMERICA. #0200-7009 • SpectraMax Paradigm Multi-Mode Microplate Reader • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Field. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Molecular Devices. Rating: 4. WARNING! HIGH VOLTAGE. 58% for the detection module and 2. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual-beam spectrophotometer. 2. Molecular Devices VMax Kinetic Microplate Reader. 1. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. View the Molecular Devices websiteThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Download PDF. i3. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 315 can't find zero order. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 18 , 42 The Förster radius (R 0 ) for the dye pair Alexa Fluor 555 and Alexa Fluor 647 (Molecular Probes) was calculated according to the spectral overlap. We recorded generalized polarization of Prodan at times points of 6 h, 12 h, 24 h, and 48 h in 96-well plate in Molecular Devices M5 instrument. Molecular Devices Spectramax M5 Microplate Reader Condition: Used, tested in working condition. If you are logged in and you do not see anything below the line, try registering an instrument or contact Technical Support with your instrument Serial number. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 标准功. After completion of the infusion, the scope and delivery devices were removed and all animals recovered well. com A. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Retrieving Each. X. 305 reference level saturation. Data were analyzed by the method of enhancement of acceptor emission. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Instrument. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 2 Software Release Notes. Molecular Devices. 00 / EA. 6. It. hgpauction. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. FlexStation II 384. 0200-7027. We would like to show you a description here but the site won’t allow us. +1 800-635-5577. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. (Agilent 5975C/G3170A TAD VL Mass selective Detector) (Agilent 7890A/G3440A GC System, With Agilent 7683B/G2913AIn apoptosis, mitochondrial outer membrane permeabilization (MOMP) triggers caspase-dependent death. Molecular Devices, LLC. For SpectraTest Validation Plate Protocol (. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. 3. Our study assesses the mosquito molecular interactions with four different fungal strains and contributes to a better understanding of mosquito antifungal immunity. Upgrades to a higher level must be purchased. 5. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. 1 Formula Reference Guide. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. Supplier: Molecular Devices. It is more inert and reduces compound interference. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. M2e, M5e or M5 readers). The lower limit of detection (LLD) was defined as the level of luciferase detectable above 3 positive standard deviations of the blank. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Monday to Friday 7am - 5pm Pacific Time. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The value of firefly luciferase luminescence/Renilla luciferase. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. ©2015 Molecular Devices, LLC 6/15 0120-1487C Printed in USA Results DNA standards ranging from 1 µg/mL to 50 pg/mL were detected using the Quant-iT PicoGreen dsDNA Assay and SpectraMax® M5 microplate reader (other fluorescence plate readers from Molecular Devices will give similar results). Keywords The chart below provides general information about the availability of on-site and depot repair options. 0; 0. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. X. 304 signal level saturation. Key applications: ELISA, nucleic acid, endotoxin testing. 6. Other technologies such as imaging. (b) Fluorescence excitation scan. Download PDF versions of the SpectraMax Microplate Reader User Guides. Step 1: Open the SoftMax Pro IMAP FP protocol. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Experimental Model and Subject Details Cell line. Molecular Devices社の製品: 蛍光マルチモードマイクロプレートリーダー SpectraMax® M seriesに関するすべての情報をご覧ください。. The Hardware Diagnostics window opens. Item Description Wavelengthrange (SpectraMaxM5andSpectraMax M5emodelsonly) 300. +1 800-635-5577. High tolerance for contaminants. SoftMax®The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and. Softmax Pro GxP Software 6. Reading fluorescence from the bottom of the well is less sensitive to the effects of fluorescence. LoadingThe SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 10. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. g. It's dual monochromators increase performance. The M5 has two PMTs. The reaction was then started by adding 20 μL of xanthine oxidase solution. Molecular Devices. Wide range of. View the Molecular Devices websiteFLIPR Calcium 4 Assay Kit. Technical specifications. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. Results Luciferase emission spectrum. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Prepare your iD series instrument for shipment. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Fluorescence Microplate Reader Comparison Chart. Heat Treatment Experiment. Uncontrolled Copy for Training and Reference Purposes Only . Three kits covering a wide range of dsDNA concentrations. view more . SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ services. View the Molecular Devices website. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different. 0 out of 5 $ 3,799. 1 - 16 of 69. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Access this by going to. Scientist - Molecular Devices) Cathy Olsen (Sr. Table of Contents. M5-G. Time-Resolved Fluorescence (M4, M5, and M5E Only) 27. When the user applies a set temperature of 37°C, the top is heated to 37. View the Molecular Devices websiteMolecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. Molecular Devices is ISO 17025 accredited† by American Association of Laboratory Accreditation (A2LA), which requires rigorous outside auditing on a regular basis so that you can be assured of the accuracy of your certificate with respect to NIST and NMI traceable standards. 2 x 10-14. HSL_EQ_017: Use and Maintenance of a Laboratory. i3, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus. 2010 2:09 PM. Published Dec 16, 2020 | Updated Dec 16, 2020. HTRF-compatible SpectraMax Multi-Mode Readers. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. With optimized reagents, option to operate the. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Published Dec 16, 2020 | Updated Dec 16, 2020. Invitrogen • LanthaScreen™ Ubiquitin and SUMO Assay Reagents User Guide Page 2 of 9 For Technical Support for this or other Drug Discovery Products, dial 760-603-7200, option 3, extension 40266 Invitrogen Corporation • 5791 Van Allen Way • Carlsbad, CA 92008 • Phone: 760 603 7200 • FAX: 760 602 6500 • IS THE RESPONSIBILITY OF POTENTIAL OFFERORS TO MONITOR THE GPE FOR ADDITIONAL INFORMMATION PERTAINING TO THIS REQUIREMENT. See SpectraMax® Microplate Reader User Manual Download Page. 004 to 146. Immunohistochemical staining and immunofluorescence staining. We will explain the software settings necessary to acquire data. Dec 16, 2020The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). After 30 min of incubation at room temperature, the plate was read on a SpectraMax M5 instrument (Molecular Devices, Sunnyvale, CA, USA) at 2 wavelengths to detect F(d) and F(a) emissions. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 4. Leadership. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide. Molecular devices spectramax™ m5 microplate reader w/ warranty (svcSpectramax® m5/m5e multimode plate reader, molecular devices Molecular devices announces next-generation spectramax minimax 300Microplate spectramax m5 molecular reader devices contract warranty svc available labx biotech laboratory readers science. See SoftMax® Pro Microplate Software User Manual Download Page how to access these in your software. Terms and Conditions Privacy Policy Online Terms of Use. Figure 2 shows the emission spectrum of firefly luciferase obtained on the SpectraMax M5. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Table 2. View the Molecular Devices websiteMolecular Devices Spectramax M5 Microplate Reader Molecular Devices Stakmax Plate Stacker Wasp Barcode Scanner Spectramax Softmax Pro 6. 26009: Use and Maintenance of Pipettes 3. 2. Please refer to the Obsolete Microplate Reader Models for more information. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Kinetic reads were performed on a Molecular Devices M5 plate reader with SoftMaxPro software. Designed to provide the simplicity, flexibility and power required for advanced data analysis. Contact Invitrogen Technical Services for instrument-specific setup guidelines. 4. The TBO absorption peaks (594 and 632 nm) were assessed via ultraviolet-visible optical absorption spectrometry (SpectraMax M5, Molecular Devices, Sunnyvale, CA, USA). ai Automated Cell Culture System, a 3D Biology Innovation Hub for Fast, Reliable Drug Discoveries. A. E (n = 16) from three independent experiments, ***P 0. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. 8. For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. Functional Description. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Uncontrolled Copy for Training and Reference Purposes Only . Microplate Reader Shelf. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different. +44-118-944-8000. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. SpectraMax QuickDrop Micro Volume Spectrophotomete. Find regional support. Plate chamber design of the SpectraMax category 1 microplate readers. The HTRF signal is defined as the emission ratio 665 nm/620 nm multiplied by 10,000. HSL_EQ_007: Use and Maintenance of a 2-8°C Refrigerator in the HPV Serology. StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. Page 1 of 8 . Sunnyvale, California -- October 5, 2017. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. . The guide also covers the software features, the specifications, and the accessories of the systems. 7°C and the bottom to 36. X. This document is copyright protected and any reproduction of this document, in whole or. Select Update Driver. 2. . Molecular Devices SpectraMax i3 - Certified with Warranty. It is always recommended to remove the calibration before performing a. In most cases we will be able to perform the repair as listed, however in rare instances, you may be required to ship the instrument into our Service Depot Facility. (Molecular Devices M5 spectra Max plate reader) for absorbance measurements to determine protein loss due to precipitation during the overnight incubation. 3. The drawing shows a standard microplate of 15 mm height covered with a lid. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader : Condition: Used. handling system from molecular devices. D. 9. 1. 0A-Sunnyvale, CA-Made in USA Molecular Devices SpectraMax® M5 The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 72 MB. Instrument. Monday to Friday 8am - 5pm GMT. 2 software with 470 nm and 520 nm absorption wavelengths for. HSL_EQ_012: Use and Maintenance of Pipettes in the HPV Serology Laboratory 3. Contact Molecular Devices technical support for equipment service that requires the removal of covers or panels. The SpectraMax® M5 Microplate Reader possesses the five modes preferred by the majority of our users providing the benefit of multiple detection modes in one platform:. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. This document is provided to customers who have purchased Molecular Devices, Inc. This item was listed in the fixed price format with a Best Offer option. We therefore gathered salient. Published Dec 16, 2020 | Updated May 19, 2023. Call 617-354-8900 for help! | Contact Us | About Us. SpectraMax ABS and ABS Plus Microplate Readers | Molecular Devices Author: Molecular Devices, LLC Subject: SpectraMax ABS and ABS Plus Microplate Readers are a compact and tunable absorbance microplate readers. The. ,. HSL_EQ_012: Use and Maintenance of Pipettes in the HPV Serology Laboratory 3. 50 / EA. 3. IQ/OQ/PM Services SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 52 0112-0115J. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingFlexStation® 3 Multi-Mode Microplate Reader. SpectraTest® Validation Package. Results Luciferase emission spectrum. Unit also passed basic testing for: Shaking, Temp, Abs, FL, Cuv Capabilities: Fluorescence (top/bottom read) Luminescence (top/bottom read) Absorbance Time-ReSpectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Gemini EM. 5. These errors are seen with SoftMax Pro 5. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up. i3x. 00 / EA. Figure 3. SpectraMax 250 SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number. , TECAN Safire 2 or M1000, BMG Pherastar, BioTek Synergy series, Molecular Devices M5 or Flexstation, PerkinElmer Envision). deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. $7,146. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance. All our used microplate readers are fully tested, serviced, and come with up to 1-year warranty and lifetime support. , Sunnyvale, CA, USA) using bottom well-scan mode where nine readings were taken per well using Ex (485 nm) and Em (572 nm) spectra. Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. DNA standards. GFP (Green Fluorescent Protein) is an extremely stable protein of 238 amino acids produced by the jellyfish Aequorea victoria 1 that fluoresces in the lower green region of the visible spectrum. book Page 1 Wednesday, July 7, 2010 2:09 PM Step 4: Incubate at room temperature for 1 hour, protected from light. 00 USD Bio-Rad C1000 Touch Thermal Cycler w/ CFX96 Optical Module $15,990. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax M5, Analyst HT or Analyst GT. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. M5,andM5e. Homogeneous Time-Resolved Fluorescence (HTRF) HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. 00 USD. book Page 1 Wednesday, July 7, 2010 2:09 PM. However, cells undergo clonogenic death even if caspases are blocked. Service and repair for your Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader • 7-day completion time: some older devices may take longer pending part availability • No risk quotation: send in your device for free– no charge for initial evaluations • Free shipping: here and back– shipping is free • Complimentary refurbishment: we. 7. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Your applications, your modes, your choice. The SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the. Best offer accepted . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 52 0112-0115J. After incubating the reaction solution at 37 °C for 20 minutes, the inhibition rate was calculated by measuring the absorbance at 450 nm using a Molecular Devices M5 Multimode Microplate Reader (USA). Also see: SoftMax® Pro Microplate Software User Manual Download Page. S. HSL_EQ_005: Use and Maintenance of a Molecular Devices M5 Plate Reader in the HPV Serology Laboratory 3. The level of Nile red staining was quantified using molecular devices M5 microplate reader using fluorescence well-scan mode (h). The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. PV444X. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) Multimode Reader Comparison Chart. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life. Read More. For any wavelengths that you plan to calibrate, click Remove Calibration. Home; Knowledge BaseMolecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. In most cases we will be able to perform the repair as listed, however in rare instances, you may be required to ship the instrument into our Service Depot Facility. Molecular Devices. PerkinElmer Plate Reader. 1. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seMolecular Devices has collaborated with various assay partners to optimize and validate homogeneous and . Photo-aged and freshly prepared His buffers were Production and Extraction of Photo-Aged Hisidine scanned across an absorbance range of 220–500 nm Photo-aged His (8 L) was produced by dissolving using a Molecular Devices M5 platereader set at approximately 40 g His per L of water (258 mM) ambient temperature, six reads per well, and with in 4. Conventional Electrophysiology. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. ( Molecular Devices ) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. After a short introduction, you will see a live demonstration of how to use SoftMax Pro GxP Software and the SpectraMax® iD3 Multi-Mode Microplate Reader to set up and acquire data using an example ELISA assay. 3. ( Molecular Devices ) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. 1. 8.